tailieunhanh - Báo cáo khoa học: " Packaging of viral RNAs in virions of adenoviruses"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Packaging of viral RNAs in virions of adenoviruses | Virology Journal BioMed Central Open Access Packaging of viral RNAs in virions of adenoviruses Li Xing1 3 and Suresh K Tikoo 1 2 Address Vectored Vaccine Program Vaccine Infectious Disease Organization University of Saskatchewan Saskatoon Saskatchewan S7N 5E3 Canada 2Vaccinology Immunotherapeutics Program School of Public Health University of Saskatchewan Saskatoon Saskatchewan S7N 5E3 Canada and 3Lady Davis Institute for Medical Research Jewish General Hospital Montreal Quebec H3T 1E2 Canada Email Li Xing - Xingli100@ Suresh KTikoo - Corresponding author Published 5 February 2009 Received 22 December 2008 Accepted 5 February 2009 Virology journal 2009 6 16 doi 1743-422X-6-16 This article is available from http content 6 1 16 2009 Xing and Tikoo licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Earlier we detected viral RNAs packaged in the porcine adenovirus PAdV -3 virions. Using Southern blot analysis we further demonstrated that the viral RNAs were predominantly packaged in CsCl purified mature capsids containing viral genome than empty intermediate capsids. Some of the packaged viral RNAs appear to be polyadenylated. Real-time reverse transcription RT -PCR analysis indicated that the copy number of the tested viral mRNAs encoding E1Bsmall and fiber proteins was less than one per full capsid. Moreover detection of viral RNA packaged in CsCl purified human adenovirus HAdV -5 virions indicates that the viral RNA packaging might be a common phenomenon in members of Adenoviridae family. Further quantitative analysis of viral protein DNA and RNA in CsCl purified mature and empty intermediate capsids of recombinant HAdV-5 expressing enhanced green fluorescent .