tailieunhanh - Báo cáo khoa học: " Consensus siRNA for inhibition of HCV genotype-4 replication"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Consensus siRNA for inhibition of HCV genotype-4 replication | Virology Journal BioMed Zentral Research Open Access Consensus siRNA for inhibition of HCV genotype-4 replication Abdel Rahman N Zekri 1 Abeer A Bahnassy2 Hanaa M Alam El-Din1 and Hosny M Salama3 Address Virology and Immunology Unit Cancer Biology Department National Cancer Institute Cairo University 1st Kasr El-Aini st Cairo Egypt 2Pathology Department National Cancer Institute Cairo University 1st Kasr El-Aini st Cairo Egypt and 3Tropical Medicine Faculty of Medicine Cairo University Kasr El-Aini st Cairo Egypt E-mail Abdel Rahman N Zekri - ncizekri@ Abeer A Bahnassy - chaya2000@ Hanaa M Alam El-Din - halam63@ Hosny M Salama - hsalama888@ Corresponding author Published 27 January 2009 Received I December 2008 Virology Journal 2009 6 13 doi 1743-422X-6-13 Accepted 27 January 2009 This article is available from http content 6 I I3 2009 Zekri et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background HCV is circulating as a heterogeneous group of quasispecies. It has been addressed that siRNA can inhibit HCV replication in-vitro using HCV clone and or replicon which have only one genotype. The current study was conducted to assess whether siRNA can inhibit different HCV genotypes with many quasispecies and to assess whether consensus siRNA have the same effect as regular siRNA. Methods We generated two chemically synthesized consensus siRNAs Z3 and Z5 which cover most known HCV genotype sequences and quasispecies using Ambium system. Highly positive HCV patient s serum with nine quasispecies was transfected in-vitro to Huh-7 cell line which supports HCV genotype-4 replication. siRNA Z3 Z5 were transfected according to Qiagen Porta-lipid .