tailieunhanh - Báo cáo y học: " Altered retinal microRNA expression profile in a mouse model of retinitis pigmentosa"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Altered retinal microRNA expression profile in a mouse model of retinitis pigmentosa. | Open Access Altered retinal microRNA expression profile in a mouse model of retinitis pigmentosa Carol J LoscherH Karsten Hokamp Paul F Kenna Alasdair C Ivens Peter Humphries Arpad PalfiH and G Jane Farrar Addresses Smurfit Institute of Genetics Trinity College Dublin College Green Dublin 2 Ireland. Wellcome Trust Genome Campus Sanger Institute Hinxton Cambridge CB10 1SA UK. H These authors contributed equally to this work. Correspondence Carol J Loscher. Email loschecj@ Published 22 November 2007 Genome Biology 2007 8 R248 doi gb-2007-8- Il-r248 The electronic version of this article is the complete one and can be found online at http 2007 8 11 R248 Received 6 July 2007 Revised 10 September 2007 Accepted 22 November 2007 2007 Loscher et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The role played by microRNAs miRs as common regulators in physiologic processes such as development and various disease states was recently highlighted. Retinitis pigmentosa RP linked to RHO which encodes rhodopsin is the most frequent form of inherited retinal degeneration that leads to blindness for which there are no current therapies. Little is known about the cellular mechanisms that connect mutations within RHO to eventual photoreceptor cell death by apoptosis. Results Global miR expression profiling using miR microarray technology and quantitative realtime RT-PCR qPCR was performed in mouse retinas. RNA samples from retina of a mouse model of RP carrying a mutant Pro347Ser RHO transgene and from wild-type retina brain and a wholebody representation prepared by pooling total RNA from eight different mouse organs exhibited notably different miR profiles. Expression of retina-specific