tailieunhanh - Báo cáo khoa học: "Molecular characterization of the Great Lakes viral hemorrhagic septicemia virus (VHSV) isolate from USA"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Molecular characterization of the Great Lakes viral hemorrhagic septicemia virus (VHSV) isolate from USA | Virology Journal BioMed Central Research Molecular characterization of the Great Lakes viral hemorrhagic septicemia virus VHSV isolate from USA Arun Ammayappan1 2 and Vikram N Vakharia 1 Address 1Center of Marine Biotechnology University of Maryland Biotechnology Institute Baltimore 701 East Pratt Street Baltimore Maryland 21202-3101 USA and 2Department of Veterinary Medicine University of Maryland College Park MD 20742 USA Email Arun Ammayappan - ammayapp@ Vikram N Vakharia - vakharia@ Corresponding author Open Access Published 25 October 2009 Received 7 September 2009 Accepted 25 October 2009 Virology Journal 2009 6 171 doi 1743-422X-6-171 This article is available from http content 6 1 171 2009 Ammayappan and Vakharia licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract__ Background Viral hemorrhagic septicemia virus VHSV is a highly contagious viral disease of fresh and saltwater fish worldwide. VHSV caused several large scale fish kills in the Great Lakes area and has been found in 28 different host species. The emergence of VHS in the Great Lakes began with the isolation of VHSV from a diseased muskellunge Esox masquinongy caught from Lake St. Clair in 2003. VHSV is a member of the genus Novirhabdovirus within the family Rhabdoviridae. It has a linear single-stranded negative-sense RNA genome of approximately II kbp with six genes. VHSV replicates in the cytoplasm and produces six monocistronic mRNAs. The gene order of VHSV is 3 -N-P-M-G-NV-L-5 . This study describes molecular characterization of the Great Lakes VHSV strain MI03GL and its phylogenetic relationships with selected European