tailieunhanh - Báo cáo khoa học: "Phylogenetic analysis and characterization of Korean orf virus from dairy goats: case report"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Phylogenetic analysis and characterization of Korean orf virus from dairy goats: case report | Virology Journal BioMed Central Open Access Case Report Phylogenetic analysis and characterization of Korean orf virus from dairy goats case report Jae-Ku Oem In-Soon Roh Kyung-Hyun Lee Kyoung-Ki Lee Hye-Ryoung Kim Young-Hwa Jean and O-Soo Lee Address Animal Disease Diagnostic Center National Veterinary Research and Quarantine Service 480 Anyang-6-Dong Anyang 430-824 Republic of Korea Email Jae-Ku Oem - jku0622@ In-Soon Roh - isroh@ Kyung-Hyun Lee - leeh0115@ Kyoung- Ki Lee - leekk@ Hye-Ryoung Kim - kimhr@ Young-Hwa Jean - Jean@ O-Soo Lee - leeosoo@ Corresponding author Published 16 October 2009 Received 4 July 2009 Accepted 16 October 2009 Virology Journal 2009 6 167 doi 1743-422X-6-167 This article is available from http content 6 1 167 2009 Oem et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract An outbreak of orf virus infection in dairy goats in Korea was investigated. Suspected samples of the skin and lip of affected goats were sent to the laboratory for more exact diagnosis. Orf virus was detected by electron microscopy and viral DNA was identified by PCR. To reveal the genetic characteristics of the Korean strain ORF 09 Korea the sequences of the major envelope protein B2L and orf virus interferon resistance VIR genes were determined and then compared with published reference sequences. Phylogenetic analysis revealed that the ORF 09 Korea strain was closest to the isolates Taiping from Taiwan. This is believed to be the first report on the molecular characterization of orf virus in Korea. Background Contagious ecthyma contagious pustular dermatitis orf is a common epitheliotrophic viral disease of sheep