tailieunhanh - Báo cáo khoa học: " Production of recombinant AAV vectors encoding insulin-like growth factor I is enhanced by interaction among AAV rep regulatory sequences"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Production of recombinant AAV vectors encoding insulin-like growth factor I is enhanced by interaction among AAV rep regulatory sequences | Virology Journal BioMed Central Research Production of recombinant AAV vectors encoding insulin-like growth factor I is enhanced by interaction among AAV rep regulatory sequences Shuiliang Shi1 Scott A Mercer1 Robert Dilley1 3 and Stephen B Trippel 1 2 Open Access Address Department of Orthopaedic Surgery Indiana University School of Medicine Indianapolis IN USA 2Department of Anatomy and Cell Biology Indiana University School of Medicine Indianapolis IN USA and 3Indiana State Police Laboratory 550 West 16th Street Indianapolis IN 46202. USA Email Shuiliang Shi - shushi@ Scott A Mercer - scmercer@ Robert Dilley - rdilley@ Stephen BTrippel - strippel@ Corresponding author Published 7 January 2009 Received 29 October 2008 Accepted 7 January 2009 Virology Journal 2009 6 3 doi 1743-422X-6-3 This article is available from http content 6 1 3 2009 Shi et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract_ Background Adeno-associated virus AAV vectors are promising tools for gene therapy. Currently their potential is limited by difficulties in producing high vector yields with which to generate transgene protein product. AAV vector production depends in part upon the replication Rep proteins required for viral replication. We tested the hypothesis that mutations in the start codon and upstream regulatory elements of Rep78 68 in AAV helper plasmids can regulate recombinant AAV rAAV vector production. We further tested whether the resulting rAAV vector preparation augments the production of the potentially therapeutic transgene insulin-like growth factor I IGF-I . Results We constructed