tailieunhanh - Báo cáo khoa học: "Identification and characterization of a new E3 ubiquitin ligase in white spot syndrome virus involved in virus latency"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Identification and characterization of a new E3 ubiquitin ligase in white spot syndrome virus involved in virus latency | Virology Journal BioMed Central Research Identification and characterization of a new E3 ubiquitin ligase in white spot syndrome virus involved in virus latency Fang He1 and Jimmy Kwang 1 2 Open Access Address 1Animal Health Biotechnology Temasek Life Sciences Laboratory National University of Singapore 1 Research Link Singapore 117604 Singapore and 2Department of Microbiology Faculty of Medicine National University of Singapore Block MD4 5 Science Drive 2 Singapore 117597 Singapore Email Fang He - hefang@ Jimmy Kwang - kwang@ Corresponding author Published 17 December 2008 Received 29 August 2008 Accepted 17 December 2008 Virologyjournal 2008 5 151 doi l743-422X-5-l5l This article is available from http content 5 l l5l 2008 He and Kwang licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract White spot syndrome virus WSSV is one major pathogen in shrimp aquaculture. WSSV ORF403 is predicted to encode a protein of 64l amino acids which contains a C3H2C2 RING structure. In the presence of an E2 conjugating enzyme from shrimp WSSV403 can ubiquitinate itself in vitro indicating it can function as a viral E3 ligase. Besides WSSV403 E3 ligase can be activated by a series of E2 variants. Based on RT-PCR and Real time PCR we detected transcription of WSSV403 in the commercial specific-pathogen-free SPF shrimp suggesting its role as a latency-associated gene. Identified in yeast two-hybrid screening and verified by pull-down assays WSSV403 is able to bind to a shrimp protein phosphatase PPs which was characterized before as an interaction partner for another latent protein WSSV427. Our studies suggest that WSSV403 is a regulator of latency state of WSSV by virtue of its E3 ligase .