tailieunhanh - Báo cáo khoa học: "Passive immunization against highly pathogenic Avian Influenza Virus (AIV) strain H7N3 with antiserum generated from viral polypeptides protect poultry birds from lethal viral infection"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Passive immunization against highly pathogenic Avian Influenza Virus (AIV) strain H7N3 with antiserum generated from viral polypeptides protect poultry birds from lethal viral infection | Virology Journal BioMed Central Research Passive immunization against highly pathogenic Avian Influenza Virus AIV strain H7N3 with antiserum generated from viral polypeptides protect poultry birds from lethal viral infection Mirza Imran Shahzad1 Khalid Naeem2 Muhammad Mukhtar 1 and Azra Khanum1 Address Department of Biochemistry Pir Mehr Ali Shah Arid Agriculture University Murree Rawalpindi-46300 Pakistan and 2National Reference Laboratory for Poultry Diseases NRLPD Animal Sciences Institute National Agricultural Research Center NARC Islamabad Pakistan Email Mirza Imran Shahzad - Khalid Naeem - naeem22@ Muhammad Mukhtar - Azra Khanum - azrakhanum@ Corresponding author Open Access Published 28 November 2008 Received 10 June 2008 Accepted 28 November 2008 Virologyjournal 2008 5 144 doi l743-422X-5-l44 This article is available from http content 5 l l44 2008 Shahzad et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract_ Our studies were aimed at developing a vaccination strategy that could provide protection against highly pathogenic avian influenza virus AIV H7N3 or its variants outbreaks. A purified viral stock of highly pathogenic H7N3 isolate was lysed to isolate viral proteins by electrophresing on 12 sodium dodecyl sulfate polyacrylamide gel electrophoresis SDS-PAGE followed by their elution from gel through trituration in phosphate buffered saline PBS . Overall five isolated viral polypeptides proteins upon characterization were used to prepare hyperimmune monovalent serum against respective polypeptides independently and a mixture .