tailieunhanh - Báo cáo khoa học: " Selective receptor expression restricts Nipah virus infection of endothelial cells"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Selective receptor expression restricts Nipah virus infection of endothelial cells | Virology Journal BioMed Central Open Access Short report Selective receptor expression restricts Nipah virus infection of endothelial cells Stephanie Erbar Sandra Diederich and Andrea Maisner Address Institute of Virology Philipps University of Marburg Marburg Germany Email Stephanie Erbar - erbar@ Sandra Diederich - Andrea Maisner - maisner@ Corresponding author Published 26 November 2008 Received 30 October 2008 Accepted 26 November 2008 Virology journal 2008 5 142 doi 1743-422X-5-142 This article is available from http content 5 1 142 2008 Erbar et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract__ Nipah virus NiV is a highly pathogenic paramyxovirus that causes severe diseases in animals and humans. Endothelial cell EC infection is an established hallmark of NiV infection in vivo. Despite systemic virus spread via the vascular system EC in brain and lung are preferentially infected whereas EC in other organs are less affected. As in vivo we found differences in the infection of EC in cell culture. Only brain-derived primary or immortalized EC were found to be permissive to NiV infection. Using a replication-independent fusion assay we could show that the lack of infection in non-brain EC was due to a lack of receptor expression. The NiV entry receptors ephrinB2 EB2 or ephrinB3 were only expressed in brain endothelia. The finding that EB2 expression in previously non-permissive aortic EC rendered the cells permissive to infection then demonstrated that EB2 is not only necessary but also sufficient to allow .