tailieunhanh - Báo cáo y học: "Phenotypic and transcriptional response to selection for alcohol sensitivity in Drosophila melanogaste"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Phenotypic and transcriptional response to selection for alcohol sensitivity in Drosophila melanogaster. | Open Access Research Phenotypic and transcriptional response to selection for alcohol sensitivity in Drosophila melanogaster Tatiana V Morozova Robert RH Anholt and Trudy FC Mackay Addresses Department of Zoology North Carolina State University Raleigh NC 27695 USA. WM Keck Center for Behavioral Biology North Carolina State University Raleigh NC 27695 USA. Institute of Molecular Genetics RAS Kurchatov Square Moscow 123182 Russia. Department of Genetics North Carolina State University Raleigh NC 27695 USA. Correspondence Trudy FC Mackay. Email trudy_mackay@ Published 31 October 2007 Genome Biology 2007 8 R231 doi gb-2007-8- l0-r23l The electronic version of this article is the complete one and can be found online at http 2007 8 l0 R23l Received l May 2007 Revised 3l July 2007 Accepted 31 October 2007 2007 Morozova et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Alcoholism is a complex disorder determined by interactions between genetic and environmental risk factors. Drosophila represents a powerful model system to dissect the genetic architecture of alcohol sensitivity as large numbers of flies can readily be reared in defined genetic backgrounds and under controlled environmental conditions. Furthermore flies exposed to ethanol undergo physiological and behavioral changes that resemble human alcohol intoxication including loss of postural control sedation and development of tolerance. Results We performed artificial selection for alcohol sensitivity for 35 generations and created duplicate selection lines that are either highly sensitive or resistant to ethanol exposure along with unselected control lines. We used whole genome expression analysis to identify l .