tailieunhanh - Báo cáo khoa học: "A catalytically and genetically optimized β-lactamase-matrix based assay for sensitive, specific, and higher throughput analysis of native henipavirus entry characteristics"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: A catalytically and genetically optimized β-lactamase-matrix based assay for sensitive, specific, and higher throughput analysis of native henipavirus entry characteristics | Virology Journal BioMed Central Open Access Methodology A catalytically and genetically optimized p-lactamase-matrix based assay for sensitive specific and higher throughput analysis of native henipavirus entry characteristics Mike C Wolf1 Yao Wang1 Alexander N Freiberg4 Hector C Aguilar1 Michael R Holbrook4 and Benhur Lee 1 2 3 Address Department of Microbiology Immunology and Molecular Genetics UCLA Los Angeles CA USA 90095 2Department of Pathology and Laboratory Medicine UCLA Los Angeles CA USA 90095 3UCLA AIDS Institute UCLA Los Angeles CA USA 90095 and 4Department of Pathology University of Texas Medical Branch UTMB Galveston TX USA 77555 Email Mike C Wolf - mikewolf@ Yao Wang - wangyao@ Alexander N Freiberg - anfreibe@ Hector C Aguilar - haguilar@ Michael R Holbrook - mrholbro@ Benhur Lee - bleebhl@ Corresponding author Published 31 July 2009 Received 3 July 2009 Virology Journal 2009 6 119 doi 1743-422X-6-119 Accepted 31 July 2009 This article is available from http content 6 1 1 19 2009 Wolf et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Nipah virus NiV and Hendra virus HeV are the only paramyxoviruses requiring Biosafety Level 4 BSL-4 containment. Thus study of henipavirus entry at less than BSL-4 conditions necessitates the use of cell-cell fusion or pseudotyped reporter virus assays. Yet these surrogate assays may not fully emulate the biological properties unique to the virus being studied. Thus we developed a henipaviral entry assay based on a p-lactamase-Nipah Matrix Pla-M fusion protein. We first codon-optimized the bacterial pia and the NiV-M genes to ensure efficient expression in mammalian cells. The pla-M construct .