tailieunhanh - Báo cáo y học: "Genetic subtraction profiling identifies genes essential for Arabidopsis reproduction and reveals interaction between the female gametophyte and the maternal sporophyte"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Genetic subtraction profiling identifies genes essential for Arabidopsis reproduction and reveals interaction between the female gametophyte and the maternal sporophyte. | Open Access Research Genetic subtraction profiling identifies genes essential for Arabidopsis reproduction and reveals interaction between the female gametophyte and the maternal sporophyte Amal J Johnston Patrick Meier Jacqueline Gheyselinck Samuel EJ Wuest Michael Federer Edith Schlagenhauf Jorg D Becker and Ueli Grossniklaus Addresses Institute of Plant Biology and Zurich-Basel Plant Science Center Zollikerstrasse University of Zurich CH-8008 Zurich Switzerland. Centro de Biologia do Desenvolvimento Instituto Gulbenkian de Ciência Rua da Quinta Grande PT-2780-156 Oeiras Portugal. Current address Institute of Plant Sciences and Zurich-Basel Plant Science Center ETH Zurich Universitatstrasse CH-8092 Zurich Switzerland. These authors contributed equally to this work. Correspondence Ueli Grossniklaus. Email grossnik@ Published 3 October 2007 Genome Biology 2007 8 R204 doi gb-2007-8- l0-r204 The electronic version of this article is the complete one and can be found online at http 2007 8 10 R204 Received 9 February 2007 Revised 10 September 2007 Accepted 3 October 2007 2007 Johnston et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The embryo sac contains the haploid maternal cell types necessary for double fertilization and subsequent seed development in plants. Large-scale identification of genes expressed in the embryo sac remains cumbersome because of its inherent microscopic and inaccessible nature. We used genetic subtraction and comparative profiling by microarray between the Arabidopsis thaliana wild-type and a sporophytic mutant lacking an embryo sac in order to identify embryo sac expressed genes in this model organism. The influences of the embryo sac