tailieunhanh - Báo cáo y học: "Making the most of high-throughput protein-interaction data"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Making the most of high-throughput protein-interaction data. | Opinion Making the most of high-throughput protein-interaction data Robert Gentleman and Wolfgang Huber Addresses Fred Hutchinson Cancer Research Center Seattle WA 98109 USA. European Bioinformatics Institute European Molecular Biology Laboratory Cambridge CB10 1SD UK. Published 2 November 2007 Genome Biology 2007 8 112 doi gb-2007-8-10-112 The electronic version of this article is the complete one and can be found online at http 2007 8 10 112 2007 BioMed Central Ltd Abstract We review the estimation of coverage and error rate in high-throughput protein-protein interaction datasets and argue that reports of the low quality of such data are to a substantial extent based on misinterpretations. Probabilistic statistical models and methods can be used to estimate properties of interest and to make the best use of the available data. Most protein functions involve their interaction with other molecules often with other proteins in the assembly of operational complexes. A better understanding of protein interactions is fundamental to the study of biological systems. As many drugs act on proteins it is also a prerequisite for understanding intended and unintended drug effects. Over the past few years a number of large-scale experiments have set out to map protein interactions systematically 1-15 . While there is interest in combining the resulting data there appear to be substantial discrepancies between experiments and evaluation studies have reported large error rates lack of overlap and apparent contradictions between the different datasets 16-21 . The purpose of this article is to critically assess the methodology used to analyze protein-interaction datasets. When interpreting individual experiments or combining datasets from different experiments we need to consider three questions first what do we want to know and which experiments provide data that can be used to answer our questions second which types of protein interactions were assayed