tailieunhanh - Báo cáo y học: " Effect of erythropoietin on level of circulating endothelial progenitor cells and outcome in patients after acute ischemic stroke"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Effect of erythropoietin on level of circulating endothelial progenitor cells and outcome in patients after acute ischemic stroke. | Yip et al. Critical Care 2011 15 R40 http content 15 1 R40 KS CRITICAL CARE RESEARCH Open Access Effect of erythropoietin on level of circulating endothelial progenitor cells and outcome in patients after acute ischemic stroke 3 1 3 3 24 Hon-Kan Yip 1 Tzu-Hsien Tsai Hung-Sheng Lin Shu-Fang Chen Cheuk-Kwan Sun Steve Leu 1 Chun-Man Yuen5 Teng-Yeow Tan 3 Min-Yu Lan3 Chia-Wei Liou3 Cheng-Hsien Lu3 Wen-Neng Chang33 Abstract Introduction Erythropoietin EPO enhances the circulating level of endothelial progenitor cells EPCs which has been reported to be associated with prognostic outcome in ischemic stroke IS patients. The aim of this study was to evaluate the time course of circulating EPC level and the impact of EPO therapy on EPC level and clinical outcome in patients after acute IS. Methods In total 167 patients were prospectively randomized to receive either EPO therapy group 1 5 000 IU each time subcutaneously at 48 h and 72 h after acute IS or serve as placebo group 2 . The circulating level of EPCs double-stained markers CD31 CD34 E1 CD62E CD34 E2 and KDR CD34 E3 was determined using flow cytometry at 48 h and on days 7 and 21 after IS. EPC level was also evaluated once in 60 healthy volunteers. Results Circulating EPC E1 to E3 level at 48 h after IS was remarkably higher in patients than in control subjects P . At 48 h and on Day 7 after IS EPC E1 to E3 level did not differ between groups 1 and 2 all P . However by Day 21 EPC E1 to E3 level was significantly higher in group 1 than in group 2 all P . Additionally 90-day recurrent stroke rate was notably lower ingroup 1 compared with group 2 P . Multivariate analysis demonstrated that EPO therapy 95 confidence interval CI to P and EPC E3 95 CI to P levels were significantly and independently predictive of a reduced 90day major adverse neurological event MANE defined as recurrent stroke National Institutes of Health Stroke scale 8 or death .