tailieunhanh - Báo cáo khoa học: " The cross-reactivity of the enterovirus 71 to human brain tissue and identification of the cross-reactivity related fragments"

The cross-reactivity of the enterovirus 71 to human brain tissue and identification of the cross-reactivity related fragments | Jia et al. Virology Journal 2010 7 47 http content 7 1 47 VIROLOGY JOURNAL RESEARCH Open Access The cross-reactivity of the enterovirus 71 to human brain tissue and identification of the cross-reactivity related fragments 1 1 1 1 1 1 iz 2 2 Chun Shi Jia Jiang Ning Liu Wan Bo Li Chun Mei Ma Shu Zhu Lin Yi Hao Xue Zhong Gao Xiao Lin Liu Yan Feng Xu1 Lian Feng Zhang1 Chuan Qin1 Abstract Background EV71 occasionally cause a series of severe neurological symptoms including aseptic meningitis encephalitis and poliomyelitis-like paralysis. However the neurological destruction mechanism was remained to be clarified. This study described the cross reaction between EV71 induced IgG and human brain tissue. Results Cross reaction of the IgG from 30 EV71 infected patients sera to human tissues of cerebra was observed which suggested that some EV71 antigens could induce IgG cross-reactivity to human cerebra. To identify the regions of EV71 virus that containing above antigens the polypeptide of virus was divided into 19 peptides by expression in prokaryotes cell. Mouse anti-sera of these peptides was prepared and applied in immunohistochemical staining with human adult and fetus brain tissue respectively. The result indicated the 19 peptides can be classified into three groups strong cross-reactivity weak cross-reactivity and no cross-reactivity with human brain tissue according the cross reaction activity. Then the increased Blood Brain Barrier BBB permeability and permits IgG entry in neonatal mice after EV71 infection was determined. Conclusion EV71 induced IgG could enter BBB and cross-reacted with brain tissue in EV71 infected neonatal mice and then the peptides of EV71 that could induce cross-reactivity with brain tissue were identified which should be avoided in future vaccine designing. Background Human enterovirus EV71 was first described by Schim-dit et al. in 1974 1 which belonging to Picornaviridea family and has a single positive stranded .