tailieunhanh - Báo cáo y học: "Recombination analysis reveals a double recombination event in hepatitis E virus"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Recombination analysis reveals a double recombination event in hepatitis E virus | Wang et al. Virology Journal 2010 7 129 http content 7 1 129 VIROLOGY JOURNAL SHORT REPORT Open Access Recombination analysis reveals a double recombination event in hepatitis E virus Hua Wang 1 Wen Zhang 1 Bin Ni1 Hongxing Shen1 Yuyu Song1 Xiaochun Wang1 Shihe Shao1 Xiuguo Hua2 and Li Cui 2 Abstract Recombination of Hepatitis E Virus HEV has rarely been reported. In the present study phylogenetic and recombination analyses were performed on 134 complete HEV genomes. Three potentially significant recombination events including both intra-genotype and one inter-genotype were identified by recombination detection analysis. Recombination events I and II occurred intra-genotype and inter-genotype respectively among three isolates including the lineage represented by CHN-XJ-SW13 GU119961 swine isolate E067-SIJ05C AB369690 human isolate and JJT-Kan AB091394 human isolate and lead to the recombinant swine isolate swCH31 DQ450072 . Recombination event III occurred between the lineage represented by the NA1 M73218 and K52-87 L25595 which resulted in the recombinant Xingjiang-1 D11092 . Our analyses proved that that recombination could occur between human and swine HEV strains double recombination events existed in HEV and recombination event could happen within ORF2 region of HEV. These results will provide valuable hints for future research on HEV diversity. Findings Hepatitis E virus HEV a member of the genus Hepevi-rus is a non-enveloped virus with a positives-stranded RNA genome of approximately kb in length Reyes et al. 1990 . HEV is believed to be transmitted by the faecal-oral route and its infection affects primarily young adults and is generally mild 1 2 . The mortality rate of HEV infection is higher among women and hepatitis E virus infection is highly prevalent among pregnant women 3 4 . HEV and antibodies to HEV have been reportedly found in a wide variety of animals especially swine 5-8 . A hypothesis has arisen that zoonosis is involved