tailieunhanh - Analysis of the vp2 gene sequence of a new mutated mink enteritis parvovirus strain in PR China

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Analysis of the vp2 gene sequence of a new mutated mink enteritis parvovirus strain in PR China | Zuo et al. Virology Journal 2010 7 124 http content 7 1 124 VIROLOGY JOURNAL RESEARCH Open Access Analysis of the vp2 gene sequence of a new mutated mink enteritis parvovirus strain in PR China Jing Zuo 1 Jiahui Rao1 Huihui Xu1 Liming Ma1 Bo Li1 Yuping Wang1 Xuehui Cai2 Wenyu Han1 Liancheng Lei 1 and Bin Liu3 Abstract Background Mink enteritis virus MEV causes a highly contagious viral disease of mink with a worldwide distribution. MEV has a linear single-stranded negative-sense DNA with a genome length of approximately 5 000 bp. The VP2 protein is the major structural protein of the parvovirus encoded by the vp2 gene. VP2 is highly antigenic and plays important roles in determining viral host ranges and tissue tropisms. This study describes the bionomics and vp2 gene analysis of a mutated strain MEV-DL which was isolated recently in China and outlines its homologous relationships with other selected strains registered in Genbank. Results The MEV-DL strain can infect F81 cells with cytopathic effects. Pig erythrocytes were agglutinated by the MEV-DL strain. The generation of MEV-DL in F81 cells could infect mink within three months and cause a disease that was similar to that caused by wild-type MEV. A comparative analysis of the vp2 gene nucleotide nt sequence of MEV-DL showed that this was more than 99 homologous with other mink enteritis parvoviruses in Genbank. However the nucleotide residues at positions 1 065 and 1 238 in the MEV-DL strain of the vp2 gene differed from those of all the other MEV strains described previously. It is noteworthy that the mutation at the nucleotide residues position 1 238 led to Asp Gly replacement. This may lead to structural changes. A phylogenetic tree and sequence distance table were obtained which showed that the MEV-DL and ZYL-1 strains had the closest inheritance distance. Conclusions A new variation of the vp2 gene exists in the MEV-DL strain which may lead to structural changes of the VP2 protein. .