tailieunhanh - Báo cáo y học: " Gene expression variation in Down's syndrome mice allows prioritization of candidate genes"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Gene expression variation in Down's syndrome mice allows prioritization of candidate genes. | Open Access Research Gene expression variation in Down s syndrome mice allows prioritization of candidate genes Marc Sultan Ilaria Piccini Daniela Balzereit Ralf Herwig Nidhi G Saran Hans Lehrach Roger H Reeves and Marie-Laure Yaspo Addresses Max Planck Institute for Molecular Genetics 73 14195 Berlin Germany. Department of Physiology Johns Hopkins University School of Medicine 725 N. Wolfe St. Baltimore Maryland 21205 USA. McKusick-Nathans Institute of Genetic Medicine 733 Nth. Broadway Johns Hopkins University School of Medicine Baltimore Maryland 21205 USA. Correspondence Marc Sultan. Email sultan@ Published 25 May 2007 Genome Biology 2007 8 R91 doi gb-2007-8-5-r9l The electronic version of this article is the complete one and can be found online at http 2007 8 5 R91 Received 18 July 2006 Revised 23 February 2007 Accepted 25 May 2007 2007 Sultan et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Down s syndrome DS or trisomy 21 is a complex developmental disorder that exhibits many clinical signs that vary in occurrence and severity among patients. The molecular mechanisms responsible for DS have thus far remained elusive. We argue here that normal variation in gene expression in the population contributes to the heterogeneous clinical picture of DS and we estimated the amplitude of this variation in 50 mouse orthologs of chromosome 21 genes in brain regions of Ts65Dn a mouse model of DS . We analyzed the RNAs of eight Ts65Dn and eight euploid mice by real-time polymerase chain reaction. Results In pooled RNAs we confirmed that trisomic euploid gene expression ratios were close to . However we observed that inter-individual gene expression .