tailieunhanh - Báo cáo y học: "Use of monoclonal antibodies against Hendra and Nipah viruses in an antigen capture ELISA"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Use of monoclonal antibodies against Hendra and Nipah viruses in an antigen capture ELISA | Chiang et al. Virology Journal 2010 7 115 http content 7 1 115 VIROLOGY JOURNAL RESEARCH Open Access Use of monoclonal antibodies against Hendra and Nipah viruses in an antigen capture ELISA Cheng-Feng Chiang 1 Michael K Lo2 Paul A Rota2 Christina F Spiropoulou1 and Pierre E Rollin 1 Abstract Background Outbreaks of Hendra HeV and Nipah NiV viruses have been reported starting in 1994 and 1998 respectively. Both viruses are capable of causing fatal disease in humans and effecting great economical loss in the livestock industry. Results Through screening of hybridomas derived from mice immunized with Y-irradiated Nipah virus we identified two secreted antibodies one reactive with the nucleocapsid N protein and the other the phosphoprotein P of henipaviruses. Epitope mapping and protein sequence alignments between NiV and HeV suggest the last 14 amino acids of the carboxyl terminus of the N protein is the target of the anti-N antibody. The anti-P antibody recognizes an epitope in the amino-terminal half of P protein. These monoclonal antibodies were used to develop two antigen capture ELISAs one for virus detection and the other for differentiation between NiV and HeV. The lower limit of detection of the capture assay with both monoclonal antibodies was 400 pfu. The anti-N antibody was used to successfully detect NiV in a lung tissue suspension from an infected pig. Conclusion The antigen capture ELISA developed is potentially affordable tool to provide rapid detection and differentiation between the henipaviruses. Background Since their first occurrences in 1994 and 1998 respectively the Hendra HeV and Nipah NiV viruses have caused recurrent outbreaks throughout northeastern Australia and southern Asia 1-5 . Fruit bats of the genus Pteropus have been identified as the primary reservoirs of these viruses 6-9 . Thoroughbred horses and farmed pigs respectively were the intermediate hosts between the bat reservoir and humans in the initial outbreaks 10