tailieunhanh - Báo cáo y học: "GSMN-TB: a web-based genome-scale network model of Mycobacterium tuberculosis metabolism"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: GSMN-TB: a web-based genome-scale network model of Mycobacterium tuberculosis metabolism. | Open Access GSMN-TB a web-based genome-scale network model of Mycobacterium tuberculosis metabolism Dany JV Beste Tracy Hooper Graham Stewart Bhushan Bonde Claudio Avignone-Rossa Michael E Bushell Paul Wheeler Steffen Klamt Andrzej M Kierzek and Johnjoe McFadden Addresses School of Biomedical and Molecular Sciences University of Surrey Stag Hill Guildford Surrey GU2 7XH UK. Tuberculosis Research Group Veterinary Laboratories Agency Weybridge New Haw Addlestone KT15 3NB UK. Max Planck Institute for Dynamics of Complex Technical Systems Sandtorstrasse D-39106 Magdeburg Germany. These authors contributed equally to this work. Correspondence Johnjoe McFadden. Email Published 23 May 2007 Genome Biology 2007 8 R89 doi 186 gb-2007-8-5-r89 The electronic version of this article is the complete one and can be found online at http 2007 8 5 R89 Received 25 January 2007 Revised 16 April 2007 Accepted 23 May 2007 2007 Beste et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background An impediment to the rational development of novel drugs against tuberculosis TB is a general paucity of knowledge concerning the metabolism of Mycobacterium tuberculosis particularly during infection. Constraint-based modeling provides a novel approach to investigating microbial metabolism but has not yet been applied to genome-scale modeling of M. tuberculosis. Results GSMN-TB a genome-scale metabolic model of M. tuberculosis was constructed consisting of 849 unique reactions and 739 metabolites and involving 726 genes. The model was calibrated by growing Mycobacterium bovis bacille Calmette Guérin in continuous culture and steady-state growth parameters were measured. Flux balance analysis was