tailieunhanh - Báo cáo y học: " Analysis of 14 BAC sequences from the Aedes aegypti genome: a benchmark for genome annotation and assembly"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Analysis of 14 BAC sequences from the Aedes aegypti genome: a benchmark for genome annotation and assembly. | Research Open Access Analysis of 14 BAC sequences from the Aedes aegypti genome a benchmark for genome annotation and assembly Neil F LoboH Kathy S Campbell Daniel Thaner Becky deBruyn Hean Koo William M Gelbart Brendan J Loftus David W Severson and Frank H Collins Addresses Center for Global Health and Infectious Diseases Department of Biological Sciences University of Notre Dame Notre Dame IN 46556-0369 USA. Harvard University Cambridge MA 02138 USA. TIGR Rockville MD 20850 USA. H These authors contributed equally to this work. Correspondence Neil F Lobo. Email nlobo@ Published 22 May 2007 Genome Biology 2007 8 R88 doi 186 gb-2007-8-5-r88 The electronic version of this article is the complete one and can be found online at http 2007 8 5 R88 Received 21 December 2006 Revised 4 April 2007 Accepted 22 May 2007 2007 Lobo et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Aedes aegypti is the principal vector of yellow fever and dengue viruses throughout the tropical world. To provide a set of manually curated and annotated sequences from the Ae. aegypti genome 14 mapped bacterial artificial chromosome BAC clones encompassing Mb were sequenced assembled and manually annotated using a combination of computational genefinding expressed sequence tag EST matches and comparative protein homology. PCR and sequencing were used to experimentally confirm expression and sequence of a subset of these transcripts. Results Of the 51 manual annotations 50 and 43 demonstrated a high level of similarity to Anopheles gambiae and Drosophila melanogaster genes respectively. Ten of the 12 BAC sequences with more than one annotated gene exhibited synteny with the A. gambiae genome. Putative