tailieunhanh - Báo cáo y học: " Monitoring of gene knockouts: genome-wide profiling of conditionally essential genes"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Monitoring of gene knockouts: genome-wide profiling of conditionally essential genes. | Open Access Method Monitoring of gene knockouts genome-wide profiling of conditionally essential genes Lisa K Smith Maria J Gomez Konstantin Y Shatalin Hyunwoo Lee and Alexander A Neyfakh Addresses Center for Pharmaceutical Biotechnology University of Illinois Chicago Illinois 60607 USA. Current address Department of Biochemistry New York University School of Medicine New York New York 10016 USA. Deceased 20 April 2006 . Correspondence Hyunwoo Lee. Email hlee31@ Published 22 May 2007 Genome Biology 2007 8 R87 doi 186 gb-2007-8-5-r87 The electronic version of this article is the complete one and can be found online at http 2007 8 5 R87 Received 12 December 2006 Revised 5 March 2007 Accepted 22 May 2007 2007 Smith et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. We have developed a new microarray-based genetic technique named MGK Monitoring of Gene Knockouts for genome-wide identification of conditionally essential genes. MGK identified bacterial genes that are critical for fitness in the absence of aromatic amino acids and was further applied to identify genes whose inactivation causes bacterial cell death upon exposure to the bacteriostatic antibiotic chloramphenicol. Our findings suggest that MGK can serve as a robust tool in functional genomics studies. Background A major aim of modern biology is to establish a functional framework that relates genes and their products to biologic effects. Although much progress has been made in addressing this challenge large gaps remain in our understanding of the function and purpose of many genes in even the most well studied model organisms. For instance only 54 of Escherichia coli genes have currently been functionally characterized based on .

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