tailieunhanh - Báo cáo y học: "Characterizing the expression of the human olfactory receptor gene family using a novel DNA microarray"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Characterizing the expression of the human olfactory receptor gene family using a novel DNA microarray. | Open Access Research Characterizing the expression of the human olfactory receptor gene family using a novel DNA microarray Xiaohong Zhang Omar De la Cruz Jayant M Pinto Dan Nicolae Stuart Firestein and Yoav Gilad Addresses Department of Biological Sciences Columbia University New York NY 10027 USA. Department of Statistics University of Chicago Chicago IL 60637 USA. Department of Human Genetics University of Chicago Chicago IL 60637 USA. Department of Medicine University of Chicago Chicago IL 60637 USA. Correspondence Yoav Gilad. Email gilad@ Published 17 May 2007 Genome Biology 2007 8 R86 doi 186 gb-2007-8-5-r86 The electronic version of this article is the complete one and can be found online at http 2007 8 5 R86 Received 17 January 2007 Revised 10 April 2007 Accepted 17 May 2007 2007 Zhang et al licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Olfactory receptor OR genes were discovered more than a decade ago when Buck and Axel observed that in rats certain G-protein coupled receptors are expressed exclusively in the olfactory epithelium. Subsequently protein sequence similarity was used to identify entire OR gene repertoires of a number of mammalian species but only in mouse were these predictions followed up by expression studies in olfactory epithelium. To rectify this we have developed a DNA microarray that contains probes for most predicted human OR loci and used that array to examine OR gene expression profiles in olfactory epithelium tissues from three individuals. Results We detected expression of 437 76 human OR genes in these olfactory epithelia. Interestingly we detected widespread expression of OR pseudogenes an observation that may shed light on the .