tailieunhanh - Báo cáo y học: "A duplex real-time RT-PCR assay for detecting H5N1 avian influenza virus and pandemic H1N1 influenza virus"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: A duplex real-time RT-PCR assay for detecting H5N1 avian influenza virus and pandemic H1N1 influenza virus | Kang et al. Virology Journal 2010 7 113 http content 7 1 113 VIROLOGY JOURNAL SHORT REPORT Open Access A duplex real-time RT-PCR assay for detecting H5N1 avian influenza virus and pandemic H1N1 influenza virus Xiao-ping Kang Tao Jiangf Yong-qiang Li Fang Lin Hong Liu Guo-hui Chang Qing-yu Zhu E-de Qin Cheng-feng Qin and Yin-hui Yang Abstract A duplex real-time reverse transcriptase polymerase chain reaction RT-PCR assay was improved for simultaneous detection of highly pathogenic H5N1 avian influenza virus and pandemic H1N1 2009 influenza virus which is suitable for early diagnosis of influenza-like patients and for epidemiological surveillance. The sensitivity of this duplex real-time RT-PCR assay was TCID50 50 tissue culture infective dose for H5N1 and TCID50 for the pandemic H1N1 which was the same as that of each single-target RT-PCR for pandemic H1N1 and even more sensitive for H5N1 with the same primers and probes. No cross reactivity of detecting other subtype influenza viruses or respiratory tract viruses was observed. Two hundred and thirty-six clinical specimens were tested by comparing with single real-time RT-PCR and result from the duplex assay was 100 consistent with the results of single real-time RT-PCR and sequence analysis. Findings In March and April 2009 a novel swine-origin pandemic H1N1 influenza virus appeared and spread worldwide. The pandemic of H1N1 2009 influenza virus poses a public health threat. According to the World Health Organization WHO as of 30 April 2010 worldwide at least 17919 death cases have been reported 1 . Compared with influenza A H1N1 virus the highly pathogenic avian influenza virus H5N1 has a much higher mortality rate H5N1 has a mortality rate of more than 50 while novel pandemic H1N1 has only about 1 2 . Nevertheless pandemic H1N1 and highly pathogenic H5N1 manifest similar clinical symptoms at the early stage of infection 3 . Early detection of these two pathogens is an essential .