tailieunhanh - Báo cáo y học: "Proteome changes of lungs artificially infected with H-PRRSV and N-PRRSV by two-dimensional fluorescence difference gel electrophoresis"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Proteome changes of lungs artificially infected with H-PRRSV and N-PRRSV by two-dimensional fluorescence difference gel electrophoresis | Xiao et al. Virology Journal 2010 7 107 http content 7 1 107 VIROLOGY JOURNAL RESEARCH Open Access Proteome changes of lungs artificially infected with H-PRRSV and N-PRRSV by two-dimensional fluorescence difference gel electrophoresis Shuqi Xiao Qiwei Wang Jianyu Jia Peiqing Cong Delin Mo Xiangchun Yu Limei Qin Anning Li Yuna Niu Kongju Zhu Xiaoying Wang Xiaohong Liu and Yaosheng Chen Abstract Background Porcine reproductive and respiratory syndrome with PRRS virus PRRSV infection which causes significant economic losses annually is one of the most economically important diseases affecting swine industry worldwide. In 2006 and 2007 a large-scale outbreak of highly pathogenic porcine reproductive and respiratory syndrome PRRS happened in China and Vietnam. However little data is available on global host response to PRRSV infection at the protein level and similar approaches looking at mRNA is problematic since mRNA levels do not necessarily predict protein levels. In order to improve the knowledge of host response and viral pathogenesis of highly virulent Chinese-type PRRSV H-PRRSV and Non-high-pathogenic North American-type PRRSV strains N-PRRSV we analyzed the protein expression changes of H-PRRSV and N-PRRSV infected lungs compared with those of uninfected negative control and identified a series of proteins related to host response and viral pathogenesis. Results According to differential proteomes of porcine lungs infected with H-PRRSV N-PRRSV and uninfected negative control at different time points using two-dimensional fluorescence difference gel electrophoresis 2D-DIGE and mass spectrometry identification 45 differentially expressed proteins DEPs were identified. These proteins were mostly related to cytoskeleton stress response and oxidation reduction or metabolism. In the protein interaction network constructed based on DEPs from lungs infected with H-PRRSV HSPA8 ARHGAP29 and NDUFS1 belonged to the most central proteins whereas DDAH2 .