tailieunhanh - Báo cáo y học: "cis-Decoder discovers constellations of conserved DNA sequences shared among tissue-specific enhancers"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: cis-Decoder discovers constellations of conserved DNA sequences shared among tissue-specific enhancers. | Open Access Method cis-Decoder discovers constellations of conserved DNA sequences shared among tissue-specific enhancers Thomas Brody Wayne Rasband Kevin Baler Alexander Kuzin Mukta Kundu and Ward F Odenwald Addresses Neural Cell-Fate Determinants Section NINDS NIH Bethesda MD 20892 USA. Office of Scientific Director IRP NIMH NIH Bethesda MD 20892 USA. Correspondence Thomas Brody. Email brodyt@. Ward F Odenwald. Email ward@ Published 9 May 2007 Genome Biology 2007 8 R75 doi 186 gb-2007-8-5-r75 The electronic version of this article is the complete one and can be found online at http 2007 8 5 R75 Received 29 September 2006 Revised 18 December 2006 Accepted 9 May 2007 2007 Brody et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract A systematic approach is described for analysis of evolutionarily conserved c s-regulatory DNA using c s-Decoder a tool for discovery of conserved sequence elements that are shared between similarly regulated enhancers. Analysis of 2 086 conserved sequence blocks CSBs identified from 135 characterized enhancers reveals most CSBs consist of shorter overlapping adjacent elements that are either enhancer type-specific or common to enhancers with divergent regulatory behaviors. Our findings suggest that enhancers employ overlapping repertoires of highly conserved core elements. Background Tissue-specific coordinate gene expression requires multiple inputs that involve dynamic interactions between sequence specific DNA-binding transcription factors and their target DNAs. The enhancer or cis-regulatory module is the focal point of integration for many of these regulatory events. Enhancers which usually span to kb contain clusters of .