tailieunhanh - Báo cáo y học: "The value of correlation of serum 20S proteasome concentration and percentage of lymphocytic apoptosis in critically ill patients: a prospective observational study"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: The value of correlation of serum 20S proteasome concentration and percentage of lymphocytic apoptosis in critically ill patients: a prospective observational study. | Yousef et al. Critical Care 2010 14 R215 http content 14 6 R215 KS CRITICAL CARE RESEARCH Open Access The value of correlation of serum 20S proteasome concentration and percentage of lymphocytic apoptosis in critically ill patients a prospective observational study Ayman A Yousef1 Ghada A Suliman2 Maaly M Mabrouk2 Abstract Introduction Sepsis in critically ill patients is almost associated with bad prognosis and its early detection may improve the prognosis. However it is difficult to monitor the immunological state of these patients depending on the traditional markers of infection or inflammatory mediators. Accelerated lymphocyte death may reflect good idea about the prognosis especially when combined with 20S proteasome determinations a recently discovered marker for muscle degradation in patients with sepsis. The hypothesis of the present study is to evaluate the role of serum 20S proteasome at early diagnosis of sepsis and its correlation with lymphocyte apoptosis to predict prognosis and consequently the early interference in critically ill patients suffering from a broad range of diseases in the intensive care unit. Methods Sixty-seven critically ill adult intensive care patients were divided into two groups 32 septic critically ill patients sepsis group and 35 non-septic critically ill patients non-sepsis group in addition to 33 apparently healthy subjects from the out patient clinic control group . Patients were tested for serum values of 20S proteasome using ELISA and for percentage of lymphocyte death using annexin V and 7-aminoactinomycin D dye by flow cytometry. Results Measured median value of serum 20S proteasome was significantly higher in septic patients compared with both the non-septic and control groups. A significant increase in the percentage of apoptotic lymphocytes was detected in septic patients when compared with the non-sepsis and control groups. The correlation of both 20S proteasome and percentage of apoptotic lymphocytes .