tailieunhanh - Báo cáo y học: "Decline of nucleotide excision repair capacity in aging Caenorhabditis elegan"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Decline of nucleotide excision repair capacity in aging Caenorhabditis elegans. | Open Access Research Decline of nucleotide excision repair capacity in aging Caenorhabditis elegans Joel N Meyer Windy A Boyd Gregory A Azzam Astrid C Haugen Jonathan H Freedman and Bennett Van Houten Addresses Laboratory of Molecular Genetics National Institute of Environmental Health Sciences Alexander Drive Research Triangle Park NC 27709 USA. Taboratory of Molecular Toxicology National Institute of Environmental Health Sciences Alexander Drive Research Triangle Park NC27709 USA. Correspondence Bennett Van Houten. Email vanhout1@ Published I May 2007 Genome Biology 2007 8 R70 doi I86 gb-2007-8-5-r70 The electronic version of this article is the complete one and can be found online at http 2007 8 5 R70 Received 11 July 2006 Revised 3 November 2006 Accepted 1 May 2007 2007 Meyer et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Caenorhabditis elegans is an important model for the study of DNA damage and repair related processes such as aging neurodegeneration and carcinogenesis. However DNA repair is poorly characterized in this organism. We adapted a quantitative polymerase chain reaction assay to characterize repair of DNA damage induced by ultraviolet type C UVC radiation in C. elegans and then tested whether DNA repair rates were affected by age in adults. Results UVC radiation induced lesions in young adult C. elegans with a slope of to lesions per 10 kilobases of DNA per 100 J m2 in both nuclear and mitochondrial targets. LI and dauer larvae were more than fivefold more sensitive to lesion formation than were young adults. Nuclear repair kinetics in a well expressed nuclear gene were biphasic in nongravid adult nematodes a faster first order .