tailieunhanh - Báo cáo y học: " Maturation of the mammalian secretome"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Maturation of the mammalian secretome. | Minireview Maturation of the mammalian secretome Jeremy C Simpson Alvaro Mateos and Rainer Pepperkok Address Cell Biology and Biophysics Unit European Molecular Biology Laboratory EMBL Meyerhofstrasse 69117 Heidelberg Germany. Correspondence Jeremy C Simpson. Email simpson@. Alvaro Mateos. Email mateos@ Published 30 April 2007 Genome Biology 2007 8 211 doi gb-2007-8-4-211 The electronic version of this article is the complete one and can be found online at http 2007 8 4 211 2007 BioMed Central Ltd Abstract A recent use of quantitative proteomics to determine the constituents of the endoplasmic reticulum and Golgi complex is discussed in the light of other available methodologies for cataloging the proteins associated with the mammalian secretory pathway. The secretory pathway in mammalian cells consists of an array of membrane-bounded organelles and transport carriers through which secretory proteins move in a stepwise fashion to reach their different cellular destinations. This arrangement means that biochemical operations carried out by the pathway such as protein folding protein glycosylation or lipid biosynthesis can be compartmentalized which enables efficient and specific reactions. Because of this link between subcellular localization and function a quantitative map of the distribution of all the protein and lipid constituents of the secretory pathway the secretome is an essential first step to a comprehensive molecular understanding of how the pathway functions. For the past 30 years cell biologists and biochemists have addressed this problem by imaging immunolabeled components in intact cells and by the biochemical analysis of subcellular fractions reviewed in 1 2 . Although these approaches have generated an enormous amount of detailed knowledge on the composition of the secretory pathway the availability of the complete sequences of several eukaryotic genomes has recently enabled more systematic attempts to .