tailieunhanh - Báo cáo y học: "Endothelial progenitor cells (EPC) in sepsis with acute renal dysfunction (ARD)"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Endothelial progenitor cells (EPC) in sepsis with acute renal dysfunction (ARD). | Patschan et al. Critical Care 2011 15 R94 http content 15 2 R94 KS CRITICAL CARE RESEARCH Open Access Endothelial progenitor cells EPC in sepsis with acute renal dysfunction ARD 1 t 11 1 1 Susann A Patschan Daniel Patschan Johanna Temme Peter Korsten Johannes T Wessels Michael Koziolek1 Elvira Henze1 Gerhard A Muller1 Abstract Introduction Sepsis is characterized by systemic microvascular dysfunction. Endothelial progenitor cells EPCs are critically involved in maintaining vascular homeostasis under both physiological and pathological conditions. The aim of the present study was to analyze the endothelial progenitor cell system in patients suffering from sepsis with acute renal dysfunction. Methods Patients with newly diagnosed sepsis were recruited from the ICU in a nonrandomized prospective manner. Blood samples were obtained within the first 12 hours after the diagnosis of sepsis. For quantifying endothelial progenitor cells EPCs CD133 Flk-1 cells were enumerated by cytometric analysis. Analysis of EPC proliferation was performed by a colony-forming units CFU assay. Blood concentrations of proangiogenic mediators were measured by ELISA. Acute renal dysfunction was diagnosed according to the Acute Kidney Injury Network AKIN criteria. Depending on the overall mean creatinine concentration during the stay at the ICU patients were either assigned to a normal creatinine group or to a high creatinine group . Survival rates frequency of dialysis the simplified acute physiology score SAPS II scores and different laboratory parameters were collected used for further clinical characterization Results Circulating EPCs were significantly higher in all sepsis patients included in the study as opposed to healthy controls. Patients within the high creatinine group showed an even more pronounced EPC increase. In contrast EPC proliferation was severely affected in sepsis. Neither total circulating EPCs nor EPC proliferation differed between patients requiring .