tailieunhanh - Báo cáo y học: "Expression and intracellular localization of duck enteritis virus pUL38 protein"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Expression and intracellular localization of duck enteritis virus pUL38 protein | Xiang et al. Virology Journal 2010 7 162 http content 7 1 162 VIROLOGY JOURNAL SHORT REPORT Open Access Expression and intracellular localization of duck enteritis virus pUL38 protein 1 2 1 13 13 Jun Xiang Guangpeng Ma Shunchuan Zhang Anchun Cheng Mingshu Wang Dekang Zhu Renyong Jia3 Qihui Luo3 Zhengli Chen3 Xiaoyue Chen1 3 4 Abstract Knowledge of the intracellular location of a protein can provide useful insights into its function. Bioinformatic studies have predicted that the DEV pUL38 mainly targets the cytoplasm and nucleus. In this study we obtained anti-pUL38 polyclonal sera. These antibodies were functional in western blotting and immunofluorescence in DEV-infected duck embryo fibroblasts DEFs . pUL38 was expressed as a 51-kDa protein from 8 h post-infection onward initially showing a diffuse distribution throughout the cytoplasm and later in the nucleus. Furthermore pUL38 was found in purified virus. These results provide the first evidence of the kinetics of expression and intracellular localization of DEV pUL38. Findings Duck enteritis virus DEV is a natural pathogen of ducks and causes duck viral enteritis an acute contagious and lethal disease affecting waterfowl belonging to the family Anatidae 1 . DEV is a member of the family Herpesviridae. The DEV virion is enveloped and the genome consists of double-stranded DNA segments packaged in an icosahedral capsid 2 . The gene library of the DEV CHv strain was constructed in our laboratory and more than 72 major open reading frames ORFs were found 3 coding for enzymes structural proteins and scaffolding proteins. However the functional characteristics of most of these proteins are still unknown. To date only the kinetics of expression and intracellular location of pUL24 4 pUL31 5 6 pUL51 7 8 pUS3 9 and dUTPase 10 have been investigated. Using bioinformatic tools some putative glycoproteins and enzymes of the virus were characterized such as gC 11 gE 12 gI gD 13 and helicase pUL5 14 .