tailieunhanh - Báo cáo khoa học: "Susceptibility of turkeys to pandemic-H1N1 virus by reproductive tract insemination"

Susceptibility of turkeys to pandemic-H1N1 virus by reproductive tract insemination | Pantin-Jackwood et al. Virology Journal 2010 7 27 http content 7 1 27 VIROLOGY JOURNAL SHORT REPORT Open Access Susceptibility of turkeys to pandemic-H1N1 virus by reproductive tract insemination Mary Pantin-Jackwood Jamie L Wasilenko Erica Spackman David L Suarez David E Swayne Abstract The current pandemic influenza A H1N1 2009 pH1N1 was first recognized in humans with acute respiratory diseases in April 2009 in Mexico in swine in Canada in June 2009 with respiratory disease and in turkeys in Chile in June 2009 with a severe drop in egg production. Several experimental studies attempted to reproduce the disease in turkeys but failed to produce respiratory infection in turkeys using standard inoculation routes. We demonstrated that pH1N1 virus can infect the reproductive tract of turkey hens after experimental intrauterine inoculation causing decreased egg production. This route of exposure is realistic in modern turkey production because turkey hens are handled once a week for intrauterine insemination in order to produce fertile eggs. This understanding of virus exposure provides an improved understanding of the pathogenesis of the disease and can improve poultry husbandry to prevent disease outbreaks. Findings Because of the known susceptibility of turkeys to type A influenza viruses and the history of infection with triple reassortant viruses 1-6 when the pandemic influenza A H1N1 2009 pH1N1 emerged the possibility of turkeys becoming infected with the novel virus was investigated. However experimental challenge with pH1N1 virus by the respiratory route showed that both turkey poults and adult turkey hens were resistant to infection 7-9 but infection was produced in young turkeys by the novel intracloacal route of inoculation J. Pasick personal communication . In August 2009 pH1N1 virus was detected in two turkey breeder farms in Chile presenting drops in egg production 10 . Epidemiological investigations on the possible source of infection .