tailieunhanh - Báo cáo y học: " A mouse model to study infection against porcine circovirus type 2: viral distribution and lesions in mouse"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: A mouse model to study infection against porcine circovirus type 2: viral distribution and lesions in mouse | Li et al. Virology Journal 2010 7 158 http content 7 1 158 VIROLOGY JOURNAL RESEARCH Open Access A mouse model to study infection against porcine circovirus type 2 viral distribution and lesions in mouse w 3 3 2 2 2 r-t r -3 Jun Li Xiaoyuan Yuan Chaofan Zhang Lanfei Miao Jiaqiang Wu Jianli Shi Shaojian Xu Shangjin Cui Jinbao Wang1 2 Hongbin Ai1 Abstract Background Little information is known about viral distribution and transmission of porcine circovirus type 2 PCV2 in species other than swine. It is still a debated topic whether the PCV2 could be infected and caused clinical lesions. Our study is aimed to estimate the susceptibility of Kunming mouse to PCV2. Forty-eight 6-week-old Kunming mice were randomly divided into four groups. Group A C1-C12 was inoculated with PK-15 cell culture as a control group. Group B sPCV1-12 was inoculated orally and intramuscularly with PCV2 ml . Group C mPCV1-12 was inoculated orally and intramuscularly with PCV2 ml and a booster inoculation at days 14 and 28 after the first inoculation. Group D MixPCV1-12 was unvaccinated but released into Group C. Each group was sacrificed at 7 14 28 and 42 days post-inoculation respectively. Necropsy was checked on every mouse. Sera samples were collected for the test of PCV2 specific antibody. Tissues were collected for histopathology study and polymerase chain reaction PCR . Results The results showed that viral replication seroconversion and microscopic lesions were found in inoculated mice. Continuous existence of PCV2 viruses in lymph nodes have been confirmed by PCR which took at least seven days for the virus to be transferred into other organs from the primary interface and the diffusion to thymus had been retarded for seven days. Special PCV2 antibody could be found in PCV2 inoculation mice and was significantly higher than that in the control. Further more microscopic lesions and the main target of PCV2 focused in the lymph nodes .