tailieunhanh - Báo cáo khoa học: " Evaluation of adenovirus capsid labeling versus transgene expression"

Evaluation of adenovirus capsid labeling versus transgene expression | Li et al. Virology Journal 2010 7 21 http content 7 1 21 VIROLOGY JOURNAL SHORT REPORT Open Access Evaluation of adenovirus capsid labeling versus transgene expression 11 11 2 11 Jing Li Aiman Fatima Svetlana Komarova Hideyo Ugai Priyanka Uprety Justin C Roth Minghui Wang Robert A Oster3 David T Curiel 1 Qiana L Matthews1 4 Abstract Adenoviral vectors have been utilized for a variety of gene therapy applications. Our group has incorporated biolu-minescent fluorographic reporters and or suicide genes within the adenovirus genome for analytical and or therapeutic purposes. These molecules have also been incorporated as capsid components. Recognizing that incorporations at either locale yield potential advantages and disadvantages our report evaluates the benefits of transgene incorporation versus capsid incorporation. To this end we have genetically incorporated firefly luciferase within the early region 3 or at minor capsid protein IX and compared vector functionality by means of reporter readout. Introduction Adenoviral Ad vectors have been utilized for a variety of gene therapy applications. Their utilities are attributed to the unparalleled efficiency of gene transfer in both in vitro and in vivo contexts 1 2 . Our group along with others have incorporated imaging reporters of either bioluminescent 3 or fluorescent nature 4-8 as well as suicide genes within the adenovirus genome as a transgene for analytical and or therapeutic purposes. These molecules have also been incorporated as capsid components 3 8 . Determining the best locale for imaging mo dalities and or therapeutic genes could influence the design of Ad and conditionally replicative adenoviruses CRAds for monitoring of viral replication gene transfer and biodistribution thus improving these vectors for clinical applications. Recognizing reporter transgene incorporation or capsid incorporation yields potential advantages and disadvantages our report compares and evaluates the benefits