tailieunhanh - Báo cáo y học: "An M2e-based multiple antigenic peptide vaccine protects mice from lethal challenge with divergent H5N1 influenza viruses"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: An M2e-based multiple antigenic peptide vaccine protects mice from lethal challenge with divergent H5N1 influenza viruses | Zhao et al. Virology Journal 2010 7 9 http content 7 1 9 VIROLOGY JOURNAL RESEARCH Open Access An M2e-based multiple antigenic peptide vaccine protects mice from lethal challenge with divergent H5N1 influenza viruses z 71 2 3 1 1 2 1 S-Ị f r- Z-I 2 Guangyu Zhao Yongping Lin Lanying Du Jie Guan Shihui Sun Hongyan Sui Zhihua Kou Chris CS Chan Yan Guo1 Shibo Jiang3 Bo-Jian Zheng2 Yusen Zhou1 Abstract Background A growing concern has raised regarding the pandemic potential of the highly pathogenic avian influenza HPAI H5N1 viruses. Consequently there is an urgent need to develop an effective and safe vaccine against the divergent H5N1 influenza viruses. In the present study we designed a tetra-branched multiple antigenic peptide MAP -based vaccine designated M2e-MAP which contains the sequence overlapping the highly conserved extracellular domain of matrix protein 2 M2e of a HPAI H5N1 virus and investigated its immune responses and cross-protection against different clades of H5N1 viruses. Results Our results showed that M2e-MAP vaccine induced strong M2e-specific IgG antibody responses following 3-dose immunization of mice with M2e-MAP in the presence of Freunds or aluminium alum adjuvant. M2e-MAP vaccination limited viral replication and attenuated histopathological damage in the challenged mouse lungs. The M2e-MAP-based vaccine protected immunized mice against both clade1 VN 1194 and SZ 406H H5N1 virus challenge being able to counteract weight lost and elevate survival rate following lethal challenge of H5N1 viruses. Conclusions These results suggest that M2e-MAP presenting M2e of H5N1 virus has a great potential to be developed into an effective subunit vaccine for the prevention of infection by a broad spectrum of HPAI H5N1 viruses. Background The re-emergence of H5N1 highly pathogenic avian influenza HPAI in 2003 has caused 262 fatal cases among a total of 442 infected individuals 1 . Therefore there is an urgent need to develop .