tailieunhanh - Báo cáo y học: "5'PPP-RNA induced RIG-I activation inhibits drug-resistant avian H5N1 as well as 1918 and 2009 pandemic influenza virus replication"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: 5'PPP-RNA induced RIG-I activation inhibits drug-resistant avian H5N1 as well as 1918 and 2009 pandemic influenza virus replication | Ranjan et al. Virology Journal 2010 7 102 http content 7 1 102 VIROLOGY JOURNAL RESEARCH Open Access 5 PPP-RNA induced RIG-I activation inhibits drug-resistant avian H5N1 as well as 1918 and 2009 pandemic influenza virus replication Priya Ranjan 1 Lakshmi Jayashankar1 Varough Deyde1 Hui Zeng1 William G Davis1 Melissa B Pearce1 John B Bowzard1 Mary A Hoelscher1 Victoria Jeisy-Scott1 Mayim E Wiens1 Shivaprakash Gangappa1 Larisa Gubareva1 Adolfo García-Sastre2 Jacqueline M Katz1 Terrence M Tumpey1 Takashi Fujita 3 and Suryaprakash Sambhara 1 Abstract Background Emergence of drug-resistant strains of influenza viruses including avian H5N1 with pandemic potential 1918 and 2009 A H1N1 pandemic viruses to currently used antiviral agents neuraminidase inhibitors and M2 Ion channel blockers underscores the importance of developing novel antiviral strategies. Activation of innate immune pathogen sensor Retinoic Acid Inducible Gene-I RIG-I has recently been shown to induce antiviral state. Results In the present investigation using real time RT-PCR immunofluorescence immunoblot and plaque assay we show that 5 PPP-containing single stranded RNA 5 PPP-RNA a ligand for the intracytoplasmic RNA sensor RIG-I can be used as a prophylactic agent against known drug-resistant avian H5N1 and pandemic influenza viruses. 5 PPP-RNA treatment of human lung epithelial cells inhibited replication of drug-resistant avian H5N1 as well as 1918 and 2009 pandemic influenza viruses in a RIG-I and type 1 interferon dependant manner. Additionally 5 PPP-RNA treatment also inhibited 2009 H1N1 viral replication in vivo in mice. Conclusions Our findings suggest that 5 PPP-RNA mediated activation of RIG-I can suppress replication of influenza viruses irrespective of their genetic make-up pathogenicity and drug-sensitivity status. Background Annual influenza epidemics caused by influenza A and B viruses result in three to five million cases of severe illness with about 250 000 to 500 000