tailieunhanh - Báo cáo y học: "Synthetic rabbit-human antibody conjugate as a control in immunoassays for immunoglobulin M specific to hepatitis E virus"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Synthetic rabbit-human antibody conjugate as a control in immunoassays for immunoglobulin M specific to hepatitis E virus | Zhang et al. Virology Journal 2010 7 101 http content 7 1 101 VIROLOGY JOURNAL RESEARCH Open Access Synthetic rabbit-human antibody conjugate as a control in immunoassays for immunoglobulin M specific to hepatitis E virus Kuo Zhang Lunan Wang Min Liu Rui Zhang and Jinming Li Abstract Background In assays for anti-hepatitis E virus HEV immunoglobulin M IgM large volumes of the patient s sera cannot be easily obtained for use as a positive control. In this study we investigated an alternative chemical method in which rabbit anti-HEV IgG was conjugated with human IgM and was used as a positive control in the anti-HEV IgM assay. Rabbit anti-HEV IgG was isolated from immune sera by chromatography on protein A-Sepharose and was conjugated with human IgM by using 1-ethyl-3- 3-dimethylaminopropyl carbodiimide EDC as a crosslinker. Results The specific anti-HEV IgG antibody titer was 100 000 times that of the negative control . prebleed rabbit serum. The results of anti-HEV IgM enzyme-linked immunosobent assay showed that the antibody conjugate was similar to anti-HEV IgM antibodies produced in humans. The results of a stability experiment showed that the antibody conjugate was stable for use in external quality assessment or internal quality control trials. Conclusions We concluded that the chemically conjugated rabbit-human antibody could be used instead of the traditional serum control as a positive control in the anti-HEV IgM assay. Background Hepatitis E the major form of enterically transmitted non-A non-B hepatitis is caused by hepatitis E virus HEV 1-3 . Although an efficient cell-culture system for HEV has been developed and evaluated this system cannot be easily employed in current clinical practice because several weeks are required to culture the virus 4 . Viremia is thought to be present in the serum only during the acute phase of illness and it subsides soon after the onset of the icteric phase as HEV antibodies developing 5-7 . The .