tailieunhanh - Báo cáo khoa học: "Recombination in West Nile Virus: minimal contribution to genomic diversity"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Recombination in West Nile Virus: minimal contribution to genomic diversity | Virology Journal BioMed Central Short report Recombination in West Nile Virus minimal contribution to genomic diversity Brett E Pickett and Elliot J Lefkowitz Open Access Address Department of Microbiology University of Alabama at Birmingham Birmingham AL 35294-2170 USA Email Brett E Pickett - bpickett@ Elliot J Lefkowitz - elliotl@ Corresponding author Published 12 October 2009 Received 25 August 2009 Accepted 12 October 2009 Virology Journal 2009 6 165 doi I743-422X-6-I65 This article is available from http content 6 1 165 2009 Pickett and Lefkowitz licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Recombination is known to play a role in the ability of various viruses to acquire sequence diversity. We consequently examined all available West Nile virus WNV whole genome sequences both phylogenetically and with a variety of computational recombination detection algorithms. We found that the number of distinct lineages present on a phylogenetic tree reconstruction to be identical to the 6 previously reported. Statistically-significant evidence for recombination was only observed in one whole genome sequence. This recombination event was within the NS5 polymerase coding region. All three viruses contributing to the recombination event were originally isolated in Africa at various times with the major parent SPU116_89_B minor parent KN3829 and recombinant sequence AnMg798 belonging to WNV taxonomic lineages 2 1a and 2 respectively. This one isolated recombinant genome was out of a total of l54 sequences analyzed. It therefore does not seem likely that recombination contributes in any significant manner to the overall sequence variation within the WNV genome. Background The .