tailieunhanh - Báo cáo khoa học: " Pichinde virus induces microvascular endothelial cell permeability through the production of nitric oxide"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Pichinde virus induces microvascular endothelial cell permeability through the production of nitric oxide | Virology Journal BioMed Central Research Pichinde virus induces microvascular endothelial cell permeability through the production of nitric oxide Rebecca L Brocato and Thomas G Voss Open Access Address Department of Microbiology and Immunology Tulane University School of Medicine New Orleans LA 70112 USA Email Rebecca L Brocato - rbrocato@ Thomas G Voss - tvoss@ Corresponding author Published 8 October 2009 Received 21 August 2009 Accepted 8 October 2009 Virology Journal 2009 6 162 doi I743-422X-6-I62 This article is available from http content 6 1 162 2009 Brocato and Voss licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract This report is the first to demonstrate infection of human endothelial cells by Pichinde virus PIC . PIC infection induces an upregulation of the inducible nitric oxide synthase gene as well as an increase in detectable nitric oxide NO . PIC induces an increase in permeability in endothelial cell monolayers which can be abrogated at all measured timepoints with the addition of a nitric oxide synthase inhibitor indicating a role for NO in the alteration of endothelial barrier function. Because NO has shown antiviral activity against some viruses viral titer was measured after addition of the NO synthase inhibitor and found to have no effect in altering virus load in infected EC. The NO synthase inhibition also has no effect on levels of activated caspases induced by PIC infection. Taken together these data indicate NO production induced by Pichinde virus infection has a pathogenic effect on endothelial cell monolayer permeability. Introduction Several members of the Arenaviridae family are the agents responsible for hemorrhagic fevers. These members .