tailieunhanh - Báo cáo y học: " Diagnosis and phylogenetic analysis of Orf virus from goats in China: a case report"

Diagnosis and phylogenetic analysis of Orf virus from goats in China: a case report | Zhang et al. Virology Journal 2010 7 78 http content 7 1 78 VIROLOGY JOURNAL CASE REPORT Open Access Diagnosis and phylogenetic analysis of Orf virus from goats in China a case report Keshan Zhang Zhongxin Lu Youjun Shang Haixue Zheng Ye Jin Jijun He and Xiangtao Liu Abstract Background Orf virus ORFV is the etiological agent of contagious pustular dermatitis and is the prototype of the genus Parapoxvirus PPV . It causes a severe exanthematous dermatitis that afflicts domestic and wild small ruminants. Case presentation In the present study an outbreak of proliferative dermatitis in farmed goats. The presence of ORFV in tissue scrapings from the lips was confirmed by B2L gene polymerase chain reaction PCR amplification. The molecular characterization of the ORFV was performed using PCR amplification DNA sequencing and phylogenetic analysis of the B2L gene. Conclusion The results of this investigation indicated that the outbreak was caused by infection with an ORFV that was closely related genetically to Nantou DQ934351 which was isolated from the Tai wan province of China and Hoping EU935106 which originated from South Korea in 2008. This is the first report of the phylogenetic analysis of ORFV from goats in China. Background The ORFV is the prototype member of the genus Parapoxvirus which also includes pseudocowpox virus PCPV in cattle bovine papular stomatitis virus BPSV in cattle squirrel parapoxvirus SPPV and parapoxvirus of red deer in New Zealand PVNZ 1 2 . Contagious pustular dermatitis is a common viral skin disease that occurs in a range of species not only in wild ruminants 3 but also in humans 4-6 . Humans with immunodeficiency diseases in particular can develop serious infections 7 . The diseases caused by ORFV have worldwide distribution and have been reported from many countries 1 . The disease not only has an economic impact on farmers worldwide but also has a considerable negative effect on animal welfare. Infected animals are .

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