tailieunhanh - Báo cáo y học: " Monitoring human cytomegalovirus infection with nested PCR: comparison of positive rates in plasma and leukocytes and with quantitative PCR"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Monitoring human cytomegalovirus infection with nested PCR: comparison of positive rates in plasma and leukocytes and with quantitative PCR | Zhang et al. Virology Journal 2010 7 73 http content 7 1 73 VIROLOGY JOURNAL RESEARCH Open Access Monitoring human cytomegalovirus infection with nested PCR comparison of positive rates in plasma and leukocytes and with quantitative PCR Shu Zhang1 3 Yi-Hua Zhou2 3 Lei Li2 Yali Hu1 3 Abstract Background Human cytomegalovirus HCMV infection poses a significant health threat to immunocompromised individuals. Here we performed this study to set up a highly sensitive nested PCR method applicable for detecting HCMV infection in high-risk individuals. In this work 106 blood specimens from 66 patients with potential HCMV infection were obtained. Total DNA was extracted separately from plasma and peripheral blood leukocytes PBL of each sample. HCMV DNA was detected in parallel by nested PCR and quantitative real-time PCR qRT-PCR and the results were compared. Results Serial dilution test revealed that the detection limit of nested PCR was 180 copies ml. The nested PCR showed a higher positive rate than qRT-PCR vs. p . The positive rate of nested PCR based on PBL DNA was significantly higher than that based on plasma DNA vs. p . Of the 14 patients with serial samples 11 were positive for HCMV DNA in PBL while only 7 were positive in plasma. Moreover for each patient nested PCR using PBL DNA also detected more positive samples than that using plasma DNA. Conclusion Combined use of nested PCR with PBL DNA is highly sensitive in defining HCMV infection. This assay is particularly useful in the case of quantification not essential. Background Human cytomegalovirus HCMV an opportunistic pathogen is ubiquitously distributed in human population. The seroprevalence of HCMV in adults ranges from 55 1 in developed countries to as high as over 90 2 3 in developing countries like China. In immunocompetent persons HCMV usually just causes a latent infection with few obvious lesions but it poses a significant health threat to .