tailieunhanh - Báo cáo y học: "Hepatitis C virus genotype frequency in Isfahan province of Iran: a descriptive cross-sectional study"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Hepatitis C virus genotype frequency in Isfahan province of Iran: a descriptive cross-sectional study | Zarkesh-Esfahani et al. Virology Journal 2010 7 69 http content 7 1 69 VIROLOGY JOURNAL RESEARCH Open Access Hepatitis C virus genotype frequency in Isfahan province of Iran a descriptive cross-sectional study Sayyed H Zarkesh-Esfahani1 2 Mohammad T Kardi3 Masoud Edalati4 Abstract Background Hepatitis C is an infectious disease affecting the liver caused by the hepatitis C virus HCV . The hepatitis C virus is a small enveloped single-stranded positive sense RNA virus with a large genetic heterogeneity. Isolates have been classified into at least eleven major genotypes based on a nucleotide sequence divergence of 30-35 . Genotypes 1 2 and 3 circulate around the world while other genotypes are mainly restricted to determined geographical areas. Genotype determination of HCV is clinically valuable as it provides important information which can be used to determine the type and duration of therapy and to predict the outcome of the disease. Results Plasma samples were collected from ninety seven HCV RNA positive patients admitted to two large medical laboratory centers in Isfahan province Iran from the years 2007 to 2009. Samples from patients were subjected to HCV genotype determination using a PCR based genotyping kit. The frequency of HCV genotypes was determined as follows genotype 3a genotype 1a genotype 1b genotype 2 2 and mixed genotypes of 1a 3a 2 . Conclusion Genotype 3a is the most frequent followed by the genotype 1a genotype 1b and genotype 2 in Isfahan province Iran. Background Hepatitis C virus HCV is a small enveloped virus first isolated in 1989 and belongs to the family of flaviviridae 1 . Its genome is composed of a positive-sense singlestranded RNA encoding a polyprotein comprising structural core and envelope glycoproteins E1 and E2 and non-structural NS2 NS3a b NS4a b and NS5a b proteins. Acute HCV infection is often asymptomatic and approximately 70 of all cases progress to chronic hepatitis. This may lead to .