tailieunhanh - Báo cáo khoa học: The secretory omega-class glutathione transferase OvGST3 from the human pathogenic parasite Onchocerca volvulus

Onchocerciasis or river blindness, caused by the filarial nematodeOncho-cerca volvulus, is the second leading cause of blindness due to infectious diseases. The protective role of the omega-class glutathione transferase 3 from O. volvulus(OvGST3) against intracellular and environmental reactive oxygen species has been described previously. | ễFEBS Journal The secretory omega-class glutathione transferase OvGST3 from the human pathogenic parasite Onchocerca volvulus Eva Liebau1 Jana Hoppner1 Mareike Muhlmeister1 Cora Burmeister2 Kai Luersen1 Markus Perbandt3 Christel Schmetz4 Dietrich Buttner4 and Norbert Brattig4 1 Institute of AnimalPhysiology University of Munster Germany 2 Institute of Parasitology Justus-Liebig-University Giessen Germany 3 Institute of Biochemistry Center for Structuraland CellBiology University of Lubeck Germany 4 Bernhard Nocht Institute Hamburg Germany Keywords glutathione S-transferase nematode Onchocerca parasite Correspondence E. Liebau Institute of AnimalPhysiology University of Munster Hindenburgplatz 55 Munster 48143 Germany Fax 49 251 8321766 Tel 49 251 8321710 E-mail liebaue@ Database Additionalsequence data obtained in this study have been reported to GenBank. The original sequence data available under accession number AF203814 have been changed accordingly Received 9 February 2008 revised 22 April 2008 accepted 1 May 2008 doi Onchocerciasis or river blindness caused by the filarial nematode Onchocerca volvulus is the second leading cause of blindness due to infectious diseases. The protective role of the omega-class glutathione transferase 3 from O. volvulus OvGST3 against intracellular and environmental reactive oxygen species has been described previously. In the present study we continue our investigation of the highly stress-responsive OvGST3. Alternative splicing of two exons and one intron retention generates five different transcript isoforms that possess a spliced leader at their 5 -end indicating that the mechanism of mature mRNA production involves alternative- cis- and trans-splicing processes. Interestingly the first two exons of the ovgst3 gene encode a signal peptide before sequence identity to other omega-class glutathione transferases begins. Only the recombinant expression of the isoform that encodes the