tailieunhanh - Báo cáo khoa học: "Comparative analysis of complete nucleotide sequence of porcine reproductive and respiratory syndrome virus (PRRSV) isolates in Thailand (US and EU genotypes)"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Comparative analysis of complete nucleotide sequence of porcine reproductive and respiratory syndrome virus (PRRSV) isolates in Thailand (US and EU genotypes) | Virology Journal BioMed Central Open Access Comparative analysis of complete nucleotide sequence of porcine reproductive and respiratory syndrome virus PRRSV isolates in Thailand US and EU genotypes Alongkorn Amonsin Roongtham Kedkovid Suphasawatt Puranaveja Piya Wongyanin Sanipa Suradhat and Roongroje Thanawongnuwech Address Faculty of Veterinary Science Chulalongkorn University Henri-Dunant Road Patumwan Bangkok 10330 Thailand Email Alongkorn Amonsin - Roongtham Kedkovid - roongtham_kk@ Suphasawatt Puranaveja - psuphasawatt@ Piya Wongyanin - p_puns@ Sanipa Suradhat - Roongroje Thanawongnuwech - Corresponding author Published 16 September 2009 Received 10 August 2009 Accepted 16 September 2009 Virologyjournal 2009 6 143 doi l743-422X-6-l43 This article is available from http content 6 l l43 2009 Amonsin et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Porcine reproductive and respiratory syndrome virus PRRSV is a causative agent of Porcine Reproductive and Respiratory Syndrome PRRS . In this study the complete nucleotide sequences of the selected two Thai PRRSV isolates EU 0lCBl and US 01NP1 genotypes were determined since both isolates are the Thai prototypes. Results 01CB1 and 0l NPl contain 14 943 and 15 41 2 nucleotides respectively. The viruses compose 2 untranslated regions 5 UTR and 3 UTR and 8 open reading frames ORFs designated as ORFla ORFlb and ORF2-7. Phylogenetic analysis of full length of the viruses also showed that the 01 CBl and 01 NPl were grouped into the EU and US genotype respectively. In order to determine the genetic variation and genetic relatedness