tailieunhanh - Báo cáo khoa học: " Nucleotide identity and variability among different Pakistani hepatitis C virus isolates"

Nucleotide identity and variability among different Pakistani hepatitis C virus isolates | Virology Journal BioMed Central Research Nucleotide identity and variability among different Pakistani hepatitis C virus isolates Muhammad Idrees Sadia Butt Zunaira Awan Mahwish Aftab Bushra Khubaib Irshad-ur Rehman Madiha Akram Sobia Manzoor Haji Akbar Shazia Rafiqe and Sheikh Riazuddin Address National Centre of Excellence in Molecular Biology 87-West Canal Bank Road Thokar Niaz Baig Lahore-53700 University of the Punjab Lahore Pakistan Email Muhammad Idrees - idreeskhan96@ Sadia Butt - sadiasi@ Zunaira Awan - Mahwish Aftab - Bushra Khubaib - bushra_khubaib@ Irshad-ur Rehman - Irshad_rehman@ Madiha Akram - kiran_ak17@ Sobia Manzoor - lcianunique@ Haji Akbar - biotech_34@ Shazia Rafiqe - shaziarafique@ Sheikh Riazuddin - riaz@ Corresponding author Open Access Published 24 August 2009 Received 10 July 2009 Virology Journal 2009 6 130 doi 1743-422X-6-130 Accepted 24 August 2009 This article is available from http content 6 1 130 2009 Idrees et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The variability within the hepatitis C virus HCV genome has formed the basis for several genotyping methods and used widely for HCV genotyping worldwide. Aim The aim of the present study was to determine percent nucleotide identity and variability in HCV isolates prevalent in different geographical regions of Pakistan. Methods Sequencing analysis of the 5 noncoding region 5 -NCR of 100 HCV RNA-positive patients representing all the four provinces of Pakistan were carried out using ABI