tailieunhanh - Báo cáo khoa học: " Hepatitis B virus genotypes/subgenotypes in voluntary blood donors in Makassar, South Sulawesi, Indonesia"

Hepatitis B virus genotypes/subgenotypes in voluntary blood donors in Makassar, South Sulawesi, Indonesia | Virology Journal BioMed Central Research Hepatitis B virus genotypes subgenotypes in voluntary blood donors in Makassar South Sulawesi Indonesia Andi Utama 1 Theresia I Octavia1 Rama Dhenni1 Upik A Miskad2 Irawan Yusuf2 and Susan Tai1 Open Access Address 1Molecular Epidemiology Division Mochtar Riady Institute for Nanotechnology Lippo Karawaci Tangerang Banten 15810 Indonesia and 2Faculty of Medicine Hasanuddin University Makassar South Sulawesi 90245 Indonesia Email Andi Utama - autama@ Theresia I Octavia - tioctavia@ Rama Dhenni - rdhenni@ Upik A Miskad - Irawan Yusuf - irawanyusuf@ Susan Tai - stai@ Corresponding author Published 19 August 2009 Received 23 June 2009 Virology Journal 2009 6 128 doi I743-422X-6-I28 Accepted 19 August 2009 This article is available from http content 6 1 128 2009 Utama et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Hepatitis B virus HBV genotype appears to show varying geographic distribution. Molecular epidemiological study of HBV in particular areas in Indonesia is still limited. This study was aimed to identify the prevalence of HBV genotype subgenotype and mutations in basal core promoter BCP region in voluntary blood donors in Makassar one of the biggest cities in east part of Indonesia. A total of 214 hepatitis B surface antigen HBsAg -positive samples were enrolled in this study. HBV genotype subgenotype was identified by genotype-specific PCR method or direct sequencing of pre-S region. Mutations in BCP were identified by direct sequencing of the corresponding region. Results HBV B and HBV C were detected in and of the samples .