tailieunhanh - Báo cáo khoa học: Progressive decrease of phosphocreatine, creatine and creatine kinase in skeletal muscle upon transformation to sarcoma

In vertebrates, phosphocreatine and ATP are continuously interconverted by the reversible reaction of creatine kinase in accordance with cellular energy needs. Sarcoma tissue and its normal counterpart, creatine-rich skel-etal muscle, are good source materials to study the status of creatine and creatine kinase with the progression of malignancy. | ễFEBS Journal Progressive decrease of phosphocreatine creatine and creatine kinase in skeletal muscle upon transformation to sarcoma Subrata Patra1 Soumen Bera1 Soumya SinhaRoy1 Sarani Ghoshal1 Subhankar Ray1 Abhimanyu Basu2 Uwe Schlattner3 4 Theo Wallimann3 and Manju Ray1 1 Department of BiologicalChemistry Indian Association for the Cultivation of Science Kolkata India 2 Department of Surgery SSKM Hospital Kolkata India 3 Institute of Cell Biology ETH Zurich Switzerland 4 Laboratory of Fundamentaland Applied Bioenergetics University Joseph Fourier Grenoble France Keywords ATP cancer diagnosis creatine creatine kinase sarcoma Correspondence M. Ray Department of Biological Chemistry Indian Association for the Cultivation of Science Jadavpur Kolkata -700 032 India Fax 91 33 2473 2805 Tel 91 33 2473 4971 ext. 503 E-mail bcmr@ Website http bcmr Present address Department of Pathology CellBiology Thomas Jefferson University Philadelphia PA USA Received 26 March 2008 accepted 21 April 2008 doi In vertebrates phosphocreatine and ATP are continuously interconverted by the reversible reaction of creatine kinase in accordance with cellular energy needs. Sarcoma tissue and its normal counterpart creatine-rich skeletal muscle are good source materials to study the status of creatine and creatine kinase with the progression of malignancy. We experimentally induced sarcoma in mouse leg muscle by injecting either 3-methylcholan-threne or live sarcoma 180 cells into one hind leg. Creatine phosphocreatine and creatine kinase isoform levels decreased as malignancy progressed and reached very low levels in the final stage of sarcoma development all these parameters remained unaltered in the unaffected contralateral leg muscle of the same animal. Creatine and creatine kinase levels were also reduced significantly in frank malignant portions of human sarcoma and gastric and colonic adenocarcinoma compared with the distal .