tailieunhanh - Báo cáo y học: " Course of seasonal influenza A/Brisbane/59/07 H1N1 infection in the ferret"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Course of seasonal influenza A/Brisbane/59/07 H1N1 infection in the ferret | McBrayer et al. Virology Journal 2010 7 149 http content 7 1 149 VIROLOGY JOURNAL SHORT REPORT Open Access Course of seasonal influenza A Brisbane 59 07 H1N1 infection in the ferret Alexis McBrayer 1 Jeremy V Camp1 Ron Tapp1 Vladimir Yamshchikov1 Sheila Grimes1 Diana L Noah1 Colleen B Jonsson1 2 and Carl E Bruder 1 3 Abstract Every year influenza viruses infect approximately 5-20 of the population in the United States leading to over 200 000 hospitalizations and 36 000 deaths from flu-related complications. In this study we characterized the immune and pathological progression of a seasonal strain of H1N1 influenza virus A Brisbane 59 2007 in a ferret model. The immune response of the animals showed a dose-dependent increase with increased virus challenge as indicated by the presence of virus specific IgG IgM and neutralizing antibodies. Animals infected with higher doses of virus also experienced increasing severity of clinical symptoms and fever at 2 days post-infection DPI . Interestingly weight loss was more pronounced in animals infected with lower doses of virus compared to those infected with a higher dose these results were consistent with viral titers of swabs collected from the nares but not the throat. Analyzed specimens included nasal and throat swabs from 1 3 5 and 7 DPI as well as tissue samples from caudal lung and nasal turbinates. Viral titers of the swab samples in all groups were higher on 1 and 3 DPI and returned to baseline levels by 7 DPI. Analysis of nasal turbinates indicated presence of virus at 3 DPI in all infected groups whereas virus was only detected in the lungs of animals in the two highest dose groups. Histological analysis of the lungs showed a range of pathology such as chronic inflammation and bronchial epithelial hypertrophy. The results provided here offer important endpoints for preclinical testing of the efficacy of new antiviral compounds and experimental vaccines. Findings Every year influenza virus .