tailieunhanh - Báo cáo khoa học: Implication of the glutamine synthetase ⁄glutamate synthase pathway in conditioning the amino acid metabolism in bundle sheath and mesophyll cells of maize leaves

We investigated the role of glutamine synthetases (cytosolic GS1 and chlo-roplast GS2) and glutamate synthases (ferredoxin-GOGAT and NADH-GOGAT) in the inorganic nitrogen assimilation and reassimilation into amino acids between bundle sheath cells and mesophyll cells for the remo-bilization of amino acids during the early phase of grain filling inZea mays L. | ỊFEBS Journal Implication of the glutamine synthetase glutamate synthase pathway in conditioning the amino acid metabolism in bundle sheath and mesophyll cells of maize leaves Marie-Helene Valadier1 Ayako Yoshida2 Olivier Grandjean3 Halima Morin3 Jocelyne Kronenberger3 Stephanie Boutet1 Adeline Raballand1 Toshiharu Hase2 Tadakatsu Yoneyama4 and Akira Suzuki1 1 Unite de Nutrition Azotee des Plantes Institut Nationalde la Recherche Agronomique Versailles France 2 Institute for Protein Research Osaka University Japan 3 Laboratoire Commun de Cytologie Institut Nationalde la Recherche Agronomique Versailles France 4 Department of Applied BiologicalChemistry University of Tokyo Japan Keywords amino acid translocation compartmentation glutamine and glutamate synthesis nitrogen assimilation Zea mays L Correspondence A. Suzuki Unite de Nutrition Azotee des Plantes Institut Nationalde la Recherche Agronomique Route de St-Cyr 78026 Versailles cedex France Fax 33 1 30 83 30 96 Tel 33 1 30 83 30 87 E-mail suzuki@ Received 20 February 2008 revised 16 April2008 accepted 17 April 2008 doi We investigated the role of glutamine synthetases cytosolic GS1 and chloroplast GS2 and glutamate synthases ferredoxin-GOGAT and NADH-GOGAT in the inorganic nitrogen assimilation and reassimilation into amino acids between bundle sheath cells and mesophyll cells for the remobilization of amino acids during the early phase of grain filling in Zea mays L. The plants responded to a light dark cycle at the level of nitrate ammonium and amino acids in the second leaf upward from the primary ear which acted as the source organ. The assimilation of ammonium issued from distinct pathways and amino acid synthesis were evaluated from the diurnal rhythms of the transcripts and the encoded enzyme activities of nitrate reductase nitrite reductase GS1 GS2 ferredoxin-GOGAT NADH-GOGAT NADH-glutamate dehydrogenase and asparagine synthetase. We discerned the .

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