tailieunhanh - Báo cáo y học: " Full genome comparison and characterization of avian H10 viruses with different pathogenicity in Mink (Mustela vison) reveals genetic and functional differences in the non-structural gene"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Full genome comparison and characterization of avian H10 viruses with different pathogenicity in Mink (Mustela vison) reveals genetic and functional differences in the non-structural gene | Zohari et al. Virology Journal 2010 7 145 http content 7 1 145 VIROLOGY JOURNAL RESEARCH Open Access Full genome comparison and characterization of avian H10 viruses with different pathogenicity in Mink Mustela vison reveals genetic and functional differences in the non-structural gene Siamak Zohari 1 2 Giorgi Metreveli1 István Kiss2 Sándor Belák1 2 and Mikael Berg1 Abstract Background The unique property of some avian H10 viruses particularly the ability to cause severe disease in mink without prior adaptation enabled our study. Coupled with previous experimental data and genetic characterization here we tried to investigate the possible influence of different genes on the virulence of these H10 avian influenza viruses in mink. Results Phylogenetic analysis revealed a close relationship between the viruses studied. Our study also showed that there are no genetic differences in receptor specificity or the cleavability of the haemagglutinin proteins of these viruses regardless of whether they are of low or high pathogenicity in mink. In poly I C stimulated mink lung cells the NS1 protein of influenza A virus showing high pathogenicity in mink down regulated the type I interferon promoter activity to a greater extent than the NS1 protein of the virus showing low pathogenicity in mink. Conclusions Differences in pathogenicity and virulence in mink between these strains could be related to clear amino acid differences in the non structural 1 NS1 protein. The NS gene of mink 84 appears to have contributed to the virulence of the virus in mink by helping the virus evade the innate immune responses. Background The outbreak of severe respiratory disease in mink Mus-tela vison in 1984 was linked to an avian influenza virus of subtype H10N4. At the time this was the first known outbreak of avian influenza A virus infection in a terrestrial mammalian species 1 2 . The only possible explanation was that birds carrying the virus transmitted it via their faeces